{"repo":"nf-core/isoseq","free":true,"listed":false,"github":"https://github.com/nf-core/isoseq","clone":"git clone https://github.com/nf-core/isoseq.git","description":"Genome annotation with PacBio Iso-Seq. Takes raw subreads as input, generate Full Length Non Chemiric (FLNC) sequences and produce a bed annotation.","language":"Nextflow","stars":54,"topics":["nextflow","nf-core","pipeline","workflow","isoseq","isoseq-3","rna","ultra","tama"],"license":"MIT","category":"workflow-automation","readme_excerpt":"Introduction nf-core/isoseq is a bioinformatics best-practice analysis pipeline for Isoseq gene annotation with uLTRA and TAMA. Starting from raw isoseq subreads, the pipeline: - Generates the Circular Consensus Sequences (CSS) - Clean and polish CCS to create Full Length Non Chimeric (FLNC) reads - Maps FLNCs on the genome - Define and clean gene models The pipeline is built using Nextflow, a workflow tool to run tasks across multiple compute infrastructures in a very portable manner. It uses Docker/Singularity containers making installation trivial and results highly reproducible. The Nextflow DSL2 implementation of this pipeline uses one container per process which makes it much easier to maintain and update software dependencies. Where possible, these processes have been submitted to and installed from nf-core/modules in order to make them available to all nf-core pipelines, and to everyone within the Nextflow community! On release, automated continuous integration tests run the pipeline on a full-sized dataset on the AWS cloud infrastructure. This ensures that the pipeline runs on AWS, has sensible resource allocation defaults set to run on real-world datasets, and permits the persistent storage of results to benchmark between pipeline releases and other analysis sources. The results obtained from the full-sized test can be viewed on the nf-core website. Pipeline summary 1. Generate CCS consensuses from raw isoseq subreads ( PBCCS ) 2. Remove primer sequences from consen","default_branch":null,"files":null,"tree":[],"storefront":"/r/nf-core","claimed":false,"request_supported":{"post":"https://gitbuyer.com/r/nf-core/isoseq/request-supported","requests":0},"note":"indexed from public GitHub; nothing is for sale on this page. Clone it from GitHub. Paid listings live at /search."}